Guinea pig TXLNb (Taxilin Beta) ELISA Kit

Guinea pig TXLNb (Taxilin Beta) ELISA KitThe Guinea pig TXLNb (Taxilin Beta) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Guinea pig TXLNb. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig TXLNb and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each

The Guinea pig TXLNb (Taxilin Beta) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Guinea pig TXLNb. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig TXLNb and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Guinea pig TXLNb, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Guinea pig TXLNb. You can calculate the concentration of Guinea pig TXLNb in the samples by comparing the OD of the samples to the standard curve.