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Description
Rat IL18-BP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 40 ng/mL). Then dilute to the following concentrations: 40 ng/mL, 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 40 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 20 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotinylated detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 18 Binding Protein (IL18-BP) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 18 Binding Protein (IL18-BP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Interleukin 18 Binding Protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-18 binding protein (IL18BP) is a protein encoded by the IL18BP gene. This gene encodes an inhibitor of the proinflammatory cytokine IL-18. This protein binds to IL-18, preventing it from binding to its receptor, thereby inhibiting IL-18-induced IFN-gamma production. This protein is constitutively expressed and secreted in monocytes. Its expression can be enhanced by IFN-gamma. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.62-40 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.8 ★★★★★
Based on 2024 reviews
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Product Reviews
★★★★★ 5
Versatile with all the ports you’re looking for.
Compact and really expands your options. I was using one of the ports on the MacBook Pro M1 for a second display and the other for an external drive and then switching back and forth with the charger. This device does all that and only uses one of the ports. Plus there are multiple USB OLD STYLE still available. No external power appears to be necessary, although since I use the Mac power supply in the dedicated power in port I’d think there’d be no issue.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 7, 2026
★★★★★ 1
Good product, I suspect, if it works. (Also Marketing Needs Improvement)
UPDATE:
Nope, it's just the hub. :(
It's either defective or not a good product, which is unfortunate.
The stutter issue has been resolved, but I can still not use the dock to push an image to my secondary monitor (1080p).
I spent several hours this evening updating drivers for my Lenovo Thinkpad E15 Gen2 (AMD) without success. FYI: This laptop DOES support PD+DP through USB-C, something I confirmed before trying to go down the USB-C route in an attempt to replace my ancient Diamond USB-A dock.
An image was never sent to my second monitor, whether connected via HDMI or DP through the hub.
In all cases, the monitor IS recognized by Windows, so some information is being communicated, just not an image to the monitor.
During every attempt, when the monitor was first plugged in, responsiveness in Windows would stutter, lag, and generally respond slowly to my KB+M inputs until, eventually, the laptop caught up and was OK.
I am highly disappointed and am considering returning both products, but the USB-C dock is definitely returning.
It would be nice, but I don't need a 100W charger.
My hope of replacing my current USB-A dock is diminishing at the price point I was hoping for, so we shall see if it's up to the task of the new 2K monitor I have on the way and go from there.
Original:
Buyer be cautioned:
The Anker 565 USB-C also needs power for itself, which is evident if you think about it, but it's not stated anywhere that I could find, and it may not dawn on you until things aren't working quite right.
Lack of power could cause many issues I've read about in reviews.
The issue:
Mouse movement would stutter every 5-10 seconds.
Though Windows recognized my HDMI monitor in Device Manager/Display settings, no image was sent to it through the Anker hub.
Current theory:
So, the manufacturer's 65W USB-C charger for my laptop cannot FULLY power the hub with one connected HDMI monitor, two USBs, and Ethernet through the USB-C hub.
I did not test the two USB devices (Keyboard and headset) as there was little need once the stuttering began, which was immediate.
I have an Anker Nano 100W arriving tomorrow, and I will update the review once I've re-tested.
Suggestion:
My ask to Anker would be to *estimate* the power draw of their dock and list it somewhere with many warnings, etc.
If they wanted to go above and beyond and add averages for peripherals and the like, that would be amazing, but if not, I would understand, as I'm sure that information could become dated fast.
A power warning would have prompted me to consider the power charging situation, and I may gone with one of their docks (About a $100 increase) instead. But now that I'm annoyed, I'll purchase a 100W charger that I can use for my work laptop and elsewhere as needed. :)
They are still getting my money, but not as much.
They make amazing products, but small details like this matter to me as a consumer.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 26, 2024
★★★★★ 5
Anker 11 in 1 Docking station as a Starter.
This is an entry level addition to a PC. If more ports needed then the 14 in 1 or a powered Hub.
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Reviewed in the United States on June 3, 2026
★★★★★ 5
Works as intended
I've been into emulators lately and this is a great HDMI out with charging capabilities to go with your high speed charging brick. Doesn't work for everything, but I can attest it works with the AYN Thor and a Google Pixel 8 Pro, and presumably higher end Pixels. Both have emulators and both come thru my TV just fine. Also works with Kodi. I tried to use wired controllers, but quickly figured I'm better off going Bluetooth on that route. I haven't tried file transfer or anything because all that mattered to me was getting it to the big screen. I'd rather have a docking stand for my needs, but freedom and flexibility isn't a bad thing for this device at a decent price and cheaper than a dock.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 10, 2026
★★★★★ 5
Versatile hub adds the ports my Mac lacks
This 7-in-1 USB C hub from Anker has made it easy to connect my laptop to all the devices I need. The HDMI port consistently outputs 4K video at 60Hz, while the USB 3.0 ports and SD/microSD slots transfer files quickly without errors. I appreciate that the pass-through charging allows me to power my MacBook while using the hub, and the unit itself feels solid and well made. It's truly plug-and-play, with no drivers to install, and it greatly expands the limited port selection on modern laptops. The only minor drawback is that the hub can get a little warm under heavy use, but overall it's a reliable, convenient accessory that I feel confident recommending.
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Reviewed in the United States on March 26, 2026